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bioRxiv · 10.1101/2024.12.09.627580

The kinesin KIF3AC recycles endocytosed integrin to polarize adhesion formation towards the leading edge

Rockenbach, J. A.·Nader, G. P.·Antoku, S.·Gundersen, G. G.
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Abstract

The recycling of integrin endocytosed during focal adhesion (FA) disassembly is critical for cell migration and contributes to the polarized formation of new FAs toward the leading edge. How this occurs is unclear. Here, we sought to identify the kinesin motor protein(s) that is involved in recycling endocytosed integrin back to the plasma membrane. We show that the kinesin-2 heterodimer, KIF3AC and the Rab11 adaptor protein RCP are required for FA reformation after the disassembly of FAs in mouse and human fibroblasts. In the absence of KIF3AC, integrin does not return to the cell surface after FA disassembly and is found in the Rab11 endocytic recycling compartment. Biochemical pulldowns revealed that KIF3C associated with {beta}1 integrin in an RCP dependent fashion, but only after FA disassembly. KIF3AC knockdown inhibited cell migration, trafficking of RCP toward the leading edge, and polarized formation of FAs at the leading edge. These results show that KIF3AC promotes cell migration by recycling integrin so that it generates new FAs in a polarized fashion. SummaryThe study reveals that the heterodimeric kinesin-2 motor KIF3AC and its adaptor RCP are crucial for polarized formation of focal adhesions at the front of migrating fibroblasts. KIF3AC and RCP associate with intracellularly recycling integrin to promote its return to the cell surface after its endocytosis from disassembled focal adhesions.

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Rockenbach, J. A., Nader, G. P., Antoku, S., Gundersen, G. G.. 2024-12-12. The kinesin KIF3AC recycles endocytosed integrin to polarize adhesion formation towards the leading edge. https://doi.org/10.1101/2024.12.09.627580

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