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bioRxiv · 10.1101/2024.11.30.626188

Dynamic modulation of IRE1α-XBP1 signaling by adenovirus

Jang, Y.·Bunz, F.
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Abstract

The abundant production of foreign proteins and nucleic acids during viral infection elicits a variety of stress responses in host cells. Viral proteins that accumulate in the endoplasmic reticulum (ER) can trigger the unfolded protein response (UPR), a coordinated signaling program that culminates in the expression of downstream genes that collectively restore protein homeostasis. The model pathogen adenovirus serotype 5 (HAdV5) activates the UPR via the signaling axis formed by inositol-requiring enzyme type 1 (IRE1) and the X-box binding protein 1 (XBP1), a transcription factor required for immune function. Recent studies have suggested that IRE1-XBP1 activity supports adenovirus replication. Here, we show that HAdV5 exerted opposing effects on IRE1 and XBP1. IRE1 was activated in response to HAdV5 but the production of the XBP1 isoform, XBP1s, was post-transcriptionally blocked. The tumor suppressor p53, which is eliminated by HAdV5 after infection, inhibited IRE1 activation. The de-repression of IRE1 following the degradation of p53 conceivably reflects a novel antiviral mechanism, which HAdV5 ultimately evades by suppressing XBP1s. Our findings highlight the defective antiviral defenses in cancer cells and further illustrate the opposing mechanisms used by adenoviruses and their host cells to exert control over the UPR, a critical determinant of cell fate.

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Jang, Y., Bunz, F.. 2024-12-05. Dynamic modulation of IRE1α-XBP1 signaling by adenovirus. https://doi.org/10.1101/2024.11.30.626188

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