Cavin1 binding nanobodies reveal structural flexibility and regulated interactions of the N-terminal coiled-coil domain

Gao, Y.; Tillu, V.; Wu, Y.; Rae, J.; Hall, T. E.; Chen, K.-E.; Weeratunga, S.; Guo, Q.; Livingstone, E.; Tham, W.-H.; Parton, R. G.; Collins, B. M.

doi:10.1101/2024.11.26.625551

bioRxiv · 10.1101/2024.11.26.625551

Cavin1 binding nanobodies reveal structural flexibility and regulated interactions of the N-terminal coiled-coil domain

Gao, Y.·Tillu, V.·Wu, Y.·Rae, J.·Hall, T. E.·Chen, K.-E.·Weeratunga, S.·Guo, Q.·Livingstone, E.·Tham, W.-H.·Parton, R. G.·Collins, B. M.

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Abstract

Caveolae are abundant plasma membrane structures that regulate signalling, membrane homeostasis, and mechanoprotection. Their formation is driven by caveolins and cavins and their coordinated interactions with lipids. We have developed nanobodies against the trimeric HR1 coiled-coil domain of Cavin1. We identify specific nanobodies that do not perturb Cavin1 membrane binding and localise to caveolae when expressed in cells. The crystal structure of a nanobody HR1 complex reveals a symmetric 3:3 architecture as validated by mutagenesis. In this structure, the C-terminal half of the HR1 domain is disordered suggesting the nanobody has stabilised an open conformation previously identified as important for membrane interactions. A phosphomimic mutation in a Thr-Ser pair proximal to this region reveals selective regulation of Cavin2/Cavin3 association. These studies provide new insights into Cavin domains required for assembly of multiprotein caveolar assemblies and describe new nanobody tools for structural and functional studies of caveolae. SUMMARY STATEMENTNanobodies are reported that can label the Cavin1 protein and caveola structures in cells.

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Gao, Y., Tillu, V., Wu, Y., Rae, J., Hall, T. E., Chen, K.-E., Weeratunga, S., Guo, Q., Livingstone, E., Tham, W.-H., Parton, R. G., Collins, B. M.. 2024-11-28. Cavin1 binding nanobodies reveal structural flexibility and regulated interactions of the N-terminal coiled-coil domain. https://doi.org/10.1101/2024.11.26.625551

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