Intrinsic and extrinsic mechanisms alter neural cell fate specification in EPM1 Epilepsy

Forero, A.; Pravata, M. V.; Pipicelli, F.; Soloperto, A.; Di Matteo, F.; Bekjarova, Z.; Frenna, E.; Canafoglia, L.; Ragona, F.; Maccarrone, G.; Gonzalez Pisfil, M.; Whal-Schott, C.; Cernilogar, F. M.; Di Giaimo, R.; Cappello, S.

doi:10.1101/2024.11.20.624470

bioRxiv · 10.1101/2024.11.20.624470

Intrinsic and extrinsic mechanisms alter neural cell fate specification in EPM1 Epilepsy

Forero, A.·Pravata, M. V.·Pipicelli, F.·Soloperto, A.·Di Matteo, F.·Bekjarova, Z.·Frenna, E.·Canafoglia, L.·Ragona, F.·Maccarrone, G.·Gonzalez Pisfil, M.·Whal-Schott, C.·Cernilogar, F. M.·Di Giaimo, R.·Cappello, S.

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Abstract

The extracellular milieu, including extracellular vesicles (EVs), plays a pivotal role in brain development by regulating neural processes such as proliferation, differentiation, and migration. In this study, we sought to elucidate the pathogenesis of progressive myoclonus epilepsy type 1 (EPM1), a disease caused by mutations in the CSTB gene, using cerebral organoids (COs) derived from EPM1 patient cells. The results demonstrate that EPM1 COs display increased electrophysiological activity and a disrupted excitatory/inhibitory balance. Single-cell RNA sequencing (scRNA-seq) analysis of ventral EPM1-Cos revealed an abnormal specification of progenitor fate, with a shift toward dorsal neuron identities at the expense of inhibitory interneurons. In addition, pathological alterations in EV biogenesis and cargo were identified, including aberrant Sonic Hedgehog (SHH) signaling, which may disrupt cortical patterning. These findings suggest that both intrinsic progenitor identity shifts and extrinsic EV-mediated signaling contribute to EPM1 pathology. Our study highlights potential therapeutic strategies mediated by EVs as a novel approach to mitigate disease progression.

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Forero, A., Pravata, M. V., Pipicelli, F., Soloperto, A., Di Matteo, F., Bekjarova, Z., Frenna, E., Canafoglia, L., Ragona, F., Maccarrone, G., Gonzalez Pisfil, M., Whal-Schott, C., Cernilogar, F. M., Di Giaimo, R., Cappello, S.. 2024-11-21. Intrinsic and extrinsic mechanisms alter neural cell fate specification in EPM1 Epilepsy. https://doi.org/10.1101/2024.11.20.624470

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