Search bioRxiv⌕ Search
Home/Archive/bioRxiv

bioRxiv · 10.1101/2024.11.15.623842

Heat-Triggered Transcriptional Reprogramming in Microspores Disrupts Progression of Pollen Development in Brassica napus L.

Lohani, N.·Singh, M. B.·Bhalla, P. L.
View original source ↗Export metadata ↓

Abstract

Rising temperatures due to climate change pose significant threats to global food security, particularly by impacting crop fertility and yield. Despite the known susceptibility of male reproductive development to heat stress, the specific adaptive mechanisms and critical regulatory genes involved in the stage-specific heat stress response in crops are still not well understood. This study examines the impact of acute heat stress on pollen development in Brassica napus (Canola), an essential oilseed crop, with a focus on the uninucleate microspore stage. We demonstrate that a brief exposure to 40{degrees}C during the uninucleate microspore stage results in loss of cellular polarity, failed asymmetric cell division, loss of cytoplasm, and pollen abortion, leading to a significant decline in pollen viability. Transcriptome analysis reveals the complexity of heat-induced transcriptional reprogramming in microspores, identifying 8.1% (6,245) of the expressed genes as responsive to high temperature, with a higher degree of downregulation. Fourteen differentially expressed transcription factors (TFs) belonging to diverse TF families were identified in heat-stressed microspores, with overrepresented target genes. The differential regulation of cell cycle control genes in heat-stressed microspores supports our microscopic observations and details the transcriptional reprogramming underlying the failure of heat-stressed microspores to undergo the first and second pollen mitotic divisions (PMI and PMII), resulting in aberrant pollen development in B. napus. Heat stress disrupts key biological pathways such as transcription, mRNA processing, translational control, protein homeostasis, autophagy, phytohormone signaling, and reactive oxygen species (ROS) homeostasis. Heat stress alters the intricate network of multiple signaling pathways, potentially disrupting the regulatory pathways underlying pollen development, with evident crosstalk underscoring the complexity of the heat stress response in pollen development. These findings underscore the critical vulnerability of the microspore stage to heat stress in Canola. Understanding the identified heat-responsive genes and regulatory networks provides valuable insights for breeding climate-resilient Canola varieties, thereby contributing to global food security amidst changing climatic conditions. HighlightsO_LIAcute heat stress during microspore stage in Brassica napus disrupts pollen development. C_LIO_LIHeat stress induces complex transcriptional reprogramming in microspores, with higher degree of downregulation. C_LIO_LITranscriptional control, RNA processing, coordination of protein homeostasis and translational control is altered in heat-stressed microspores C_LIO_LIMisregulation of cell cycle control genes supports the loss of cellular polarity and failure to undergo asymmetric cell division in heat stressed microspores. C_LI

Explore related subjects

plant biologyPreprint

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Lohani, N., Singh, M. B., Bhalla, P. L.. 2024-11-17. Heat-Triggered Transcriptional Reprogramming in Microspores Disrupts Progression of Pollen Development in Brassica napus L.. https://doi.org/10.1101/2024.11.15.623842

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

bioRxiv2026

MpILR1 Hydrolyzes Jasmonate-Amino Acid Conjugates to Activate dn-iso-OPDA Signaling in Marchantia polymorpha.

Jasmonates are essential phytohormones that coordinate defense responses and developmental programs across land plants. In angiosperms, the active jasmonate ligand jasmonoyl-L-isoleucine (JA-Ile), is produced through GH3-mediated conjugation of jasmonic acid to isoleucine and JA-Ile homeostasis is further shaped by ILR1/ILL-family amidohydrolases. In contrast, the primary bioactive jasmonate ligand in bryophytes, dinor-12-oxo-phytodienoic acid (dn-iso-OPDA), is inactivated through conjugation with amino acids, raising the question of whether these conjugates constitute a reversible hormone reservoir or an irreversible catabolic end point. Although the ILR1-like family has been characterized extensively for its role in auxin and jasmonate homeostasis in angiosperms, its function in bryophytes remains basically unexplored. Here we show that MpILR1, the sole Marchantia ortholog of the ILR1/ILL family, hydrolyzes a specific subset of dn-iso-OPDA-amino acid conjugates in vivo. Loss-of-function Mpilr1 mutants exhibit enhanced accumulation of dn-iso-OPDA conjugated to hydrophobic amino acids (Val, Leu and Ile) but not to hydrophilic residues (His, Glu and Gln), demonstrating substrate-selective hydrolysis. MpILR1 hydrolytic activity is required for full dn-iso-OPDA-mediated responses, including transcriptional activation and defense against gastropod herbivory. These findings establish MpILR1 as a key positive regulator of jasmonate signaling in Marchantia polymorpha and suggest that hormone conjugation/deconjugation is an ancient regulatory mechanism evolved during plant terrestrialization.

plant biology↗
bioRxiv2026

Drought-Spec-Net: Early Tomato Drought Detection and Potential Yield-Impact Assessment Using Vis NIR Data

Drought stress significantly reduces tomato (Solanum lycopersicum L.) productivity, and early detection is critical to minimize yield losses through timely interventions. In this study, we developed Drought-Spec-Net, a hybrid 1D convolutional neural network that integrates local and global spectral feature extraction to detect early drought stress from visible and near infrared (Vis NIR) spectra data of tomato seedlings. The model was trained on 378 samples using an 80:20 train test split, with 20% of the training set reserved for validation. DroughtSpecNet outperformed the evaluated baseline and state of the art models, achieving 97% accuracy, 95% precision, 98% recall, and an F1 score of 97%. To improve the agronomic interpretation of the model outputs, predicted drought probabilities were converted into a literature-informed potential yield impact indicator using a maximum impact level of 60%. On the test set (76 samples), mapped potential yield-impact values ranged from 0% to 60%, with an average reduction of 12.97%. We also conducted an initial experiment using our greenhouse RGB dataset, collected daily from drought treated and well-watered tomato plants at West Virginia State University (WVSU). From this dataset, 44 images were selected for ilastik-based canopy segmentation, producing plant-level drought severity indices (DSI) with a mean of 0.28, median of 0.14, and range of 0.01 to 0.91. Additionally, we trained and fine-tuned a large language model (LLM) based on PLLaMA7BInstruct, called AgriLLaMA, for automated agronomic report generation from Drought-Spec-Net outputs. The generated reports summarize predicted stress levels, mapped potential yield impacts, and preliminary management considerations. This integrated approach not only improves early drought stress detection but also delivers quantitative and interpretable estimates of potential productivity losses, providing a complete framework connecting physiological stress detection to actionable agricultural outcomes.

plant biology↗
bioRxiv2026

BSA101: Unlocking Historical Mutant Collections with BSA-Seq

Forward genetics is a powerful approach for gene discovery, but identifying causal mutations becomes difficult when mutants are maintained in heterogeneous populations with uncertain pedigrees. This is exemplified by classical tasselseed (ts) mutants, which have long served as a genetic model for studying sex determination and carpel suppression. Decades of repeated outcrossing to diverse inbred lines have created substantial genetic heterogeneity, limiting the effectiveness of conventional bulked-segregant analysis sequencing (BSA-Seq). To address this, we developed a BSA-Seq framework that integrates flexible experimental designs, multiple reference genomes, and complementary statistical methods tailored for genetically heterogeneous populations. Applying this framework revealed that reference genome selection is critical for mapping success and that Euclidean distance raised to the fourth power (ED4) outperformed homozygosity mapping (HM). Furthermore, the framework enables simultaneous mapping of multiple mutations within a single population, eliminating the need for additional mapping populations. Applying this framework to 26 ts mutant stocks from the Maize Genetics Cooperation Stock Center, we successfully mapped 24 mutants to genomic intervals containing known ts genes, while the remaining mutants mapped to distinct genomic intervals, defining novel candidate regions underlying carpel suppression. Together, these results demonstrate that historical mutant collections represent an underutilized resource for gene discovery and establish a generalizable mapping strategy for unlocking their genetic potential across diverse species.

plant biology↗