AI-aided chronic mixture risk assessment along a small European river reveals multiple sites at risk and pharmaceuticals being the main risk drivers

Weichert, F. G.; Inostroza, P. A.; Ahlheim, J.; Backhaus, T.; Brack, W.; Brauns, M.; Fink, P.; Krauss, M.; Svedberg, P.; Hollert, H.

doi:10.1101/2024.11.15.623722

bioRxiv · 10.1101/2024.11.15.623722

AI-aided chronic mixture risk assessment along a small European river reveals multiple sites at risk and pharmaceuticals being the main risk drivers

Weichert, F. G.·Inostroza, P. A.·Ahlheim, J.·Backhaus, T.·Brack, W.·Brauns, M.·Fink, P.·Krauss, M.·Svedberg, P.·Hollert, H.

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Abstract

The vast amount of registered chemicals leads to a high diversity of substances occurring in the environment and the creation of new substances outpaces chemical risk assessment as well as monitoring strategies. Hence, risk assessment strategies need to be modified ensuring that they remain aligned with the rapid development and marketing of new substances. Here we performed a longitudinal chronic mixture risk assessment considering a real-world case study scenario with diverse anthropogenic impact types characterised by different land uses along a river in Central Germany. We sampled river water using large-volume solid phase extraction at six selected sampling sites. Following chemical analysis using liquid chromatography-high resolution mass spectrometry, we quantified 192 substances. For 34% of them, we obtained empirical chronic effect data for freshwater organisms. Furthermore, we used the open-source artificial intelligence (AI) model TRIDENT to predict chronic toxicity for all substances. A multi-scenario mixture risk assessment was conducted for three taxonomic groups, using the concentration-addition concept and considering various hazard and exposure scenarios. The results showed that the chronic risk estimates for all taxonomic groups were considerably higher when the empirical data was amended with data from in silico modelling. We identified hot spots of chemical pollution and our analysis indicated that fish were the most vulnerable taxonomic group, with pharmaceuticals being the most relevant risk drivers. Our study exemplifies the application of an AI model to predict chronic risk for aquatic organisms in combination with the consideration of multiple risk scenarios, that may complement future risk assessment strategies. HighlightsO_LI192 organic chemicals were quantified in six surface water samples along a river. C_LIO_LIMultiple hazard and exposure scenarios were considered in mixture risk assessment. C_LIO_LIArtificial intelligence was used to fill data gaps and predict chronic ecotoxicity. C_LIO_LIFish were identified as the most vulnerable taxonomic group for chronic toxicity. C_LIO_LIPharmaceuticals were the most prevalent mixture risk drivers. C_LI Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=81 SRC="FIGDIR/small/623722v1_ufig1.gif" ALT="Figure 1"> View larger version (32K): org.highwire.dtl.DTLVardef@1836b85org.highwire.dtl.DTLVardef@107eeaaorg.highwire.dtl.DTLVardef@1c60caaorg.highwire.dtl.DTLVardef@16995d0_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Weichert, F. G., Inostroza, P. A., Ahlheim, J., Backhaus, T., Brack, W., Brauns, M., Fink, P., Krauss, M., Svedberg, P., Hollert, H.. 2024-11-18. AI-aided chronic mixture risk assessment along a small European river reveals multiple sites at risk and pharmaceuticals being the main risk drivers. https://doi.org/10.1101/2024.11.15.623722

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