Assessment of the Genetic Diversity of Atlantic Bottlenose Dolphin (Tursiops truncatus) Strandings in the Mississippi Sound (USA)

Arick, M.; Landrau-Giovannetti, N.; Hsu, C.-Y.; Grover, C. E.; Reichley, S.; Magbanua, Z. V.; Pechanova, O.; Moore, D.; Kayal, E.; Linhoss, A.; Madrigal, T.; Peterman, M.; Ozdemir, O.; Peterson, D. G.; Solangi, M.; Lawrence, M.; Karsi, A.

doi:10.1101/2024.11.09.622766

bioRxiv · 10.1101/2024.11.09.622766

Assessment of the Genetic Diversity of Atlantic Bottlenose Dolphin (Tursiops truncatus) Strandings in the Mississippi Sound (USA)

Arick, M.·Landrau-Giovannetti, N.·Hsu, C.-Y.·Grover, C. E.·Reichley, S.·Magbanua, Z. V.·Pechanova, O.·Moore, D.·Kayal, E.·Linhoss, A.·Madrigal, T.·Peterman, M.·Ozdemir, O.·Peterson, D. G.·Solangi, M.·Lawrence, M.·Karsi, A.

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Abstract

The common bottlenose dolphin (Tursiops truncatus) is a key marine mammal species in the northern Gulf of Mexico, playing an essential role as a top predator. This study focuses on the genetic diversity and population structure of bottlenose dolphins stranded in the Mississippi Sound from 2010 to 2021. A total of 511 tissue samples (muscle, liver, lung, kidney, and brain) were collected from stranded dolphins, and mitochondrial DNA (mtDNA) was extracted for analysis. Using high-throughput sequencing methods, 417 samples were successfully amplified and sequenced, producing 386 complete mitogenomes. Genetic diversity metrics, such as nucleotide and haplotype diversity, were calculated, and population structure was inferred for both mitochondrial control region (mtCR) and whole mitogenome sequences. Using the whole mitogenome, the study identified four genetically distinct populations within the Mississippi Sound, demonstrating regional variation in dolphin populations. Notably, some individuals likely originated from populations outside the sampled area. The use of whole mitogenomes allowed for improved resolution of genetic diversity and population differentiation compared to previous studies using partial mtDNA sequences. These findings provide critical insights into the genetic structure of bottlenose dolphins in the region and highlight the value of using stranded animals for population genetic studies.

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Arick, M., Landrau-Giovannetti, N., Hsu, C.-Y., Grover, C. E., Reichley, S., Magbanua, Z. V., Pechanova, O., Moore, D., Kayal, E., Linhoss, A., Madrigal, T., Peterman, M., Ozdemir, O., Peterson, D. G., Solangi, M., Lawrence, M., Karsi, A.. 2024-11-11. Assessment of the Genetic Diversity of Atlantic Bottlenose Dolphin (Tursiops truncatus) Strandings in the Mississippi Sound (USA). https://doi.org/10.1101/2024.11.09.622766

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