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bioRxiv · 10.1101/2024.11.08.622488

NOODAI: A webserver for network-oriented multi-omics data analysis and integration pipeline

Totu, T.·Tomasoni, M.·Bolck, H. A.·Buljan, M.
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Abstract

Omics profiling has proven of great use for unbiased and comprehensive identification of key features that define biological phenotypes and underlie medical conditions. While each omics profile assists characterization of specific molecular components relevant for the studied phenotype, their joint evaluation can offer deeper insights into the overall mechanistic functioning of biological systems. Here, we introduce an approach where starting from representative traits (e.g., differentially expressed elements) obtained for each omics profile, we construct and analyze joint interaction networks. The resulting networks rely on the existing knowledge of confident interactions among biological entities. We use these maps to identify and describe central elements, which connect multiple entities characteristic for the studied phenotypes and we leverage MONET network decomposition tool in order to highlight functionally connected network modules. In order to enable broad usage of this approach, we developed the NOODAI software platform, which enables integrative omics analysis through a user-friendly interface. The analysis outcomes are presented both as raw output tables as well as high-quality summary plots and written reports. Since the MONET tool enables the use of algorithms with strong performance in identifying disease-relevant modules, the NOODAI software platform can be of a high value for the analysis of clinical multi-omics datasets. Availability and ImplementationThe platform is available as a web application freely accessible at https://omics-oracle.com. The source code is freely available from GitHub under the GPL3 license at: https://github.com/TotuTiberiu/NOODAI.

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Totu, T., Tomasoni, M., Bolck, H. A., Buljan, M.. 2024-11-11. NOODAI: A webserver for network-oriented multi-omics data analysis and integration pipeline. https://doi.org/10.1101/2024.11.08.622488

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