Single-cell atlas of the human immune system reveals sex-specific dynamics of immunosenescence

Rios, M. S.; Ripoll-Cladellas, A.; Omidi, F.; Ballouz, S.; Alquicira-Hernandez, J.; Oelen, R.; Franke, L.; van der Wijst, M. G. P.; Powell, J. E.; Mele, M.

doi:10.1101/2024.11.06.622096

bioRxiv · 10.1101/2024.11.06.622096

Single-cell atlas of the human immune system reveals sex-specific dynamics of immunosenescence

Rios, M. S.·Ripoll-Cladellas, A.·Omidi, F.·Ballouz, S.·Alquicira-Hernandez, J.·Oelen, R.·Franke, L.·van der Wijst, M. G. P.·Powell, J. E.·Mele, M.

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Abstract

Immunosenescence, or immune aging, is characterized by both changes in cell type abundance and a decline in cellular function, leading to increased susceptibility to immune-related diseases. Yet, the extent to which sex influences the dynamic composition of immune cells during immunosenescence remains unknown. Here, we use single-cell RNA sequencing in peripheral blood mononuclear cells from 982 donors to uncover the sex-specific immune aging dynamics. We reveal that aging drives sexually dimorphic compositional and transcriptional shifts, with females showing stronger immune remodeling. Female-specific shifts include the expansion of three cytotoxic CD8+ T effector memory subpopulations and inflammatory monocytes. In addition, female CD4+ central memory T cells exhibit abundance shifts in subpopulations involved in autoimmunity alongside age-associated transcriptional signatures enriched for autoimmune-related pathways. Conversely, males show an age-related expansion of a B cell subpopulation associated with an asymptomatic precursor state to chronic lymphocytic leukemia. These sex-biased, functionally distinct immune subpopulations represent sex-specific hallmarks of immune aging. Our work underscores the complexity and sexual dimorphism of immunosenescence and supports the development of sex-specific strategies to promote healthy aging. Highlights- A single-cell transcriptomic atlas of >1 million PBMCs from 982 donors uncovers sex-specific immune aging trajectories. - Aging females show greater cellular remodeling and stronger transcriptional shifts than males. - Female-specific expansions with age of cytotoxic CD8+ T effector memory cells, inflammatory monocytes, and CD4+ T helper 22 cells reflect a systemic shift toward a pro-inflammatory, self-reactive immune state. - Male-specific B cell shifts reveal expansion of CD5+ clonal subpopulations linked to leukemic precursor states. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=158 SRC="FIGDIR/small/622096v2_ufig1.gif" ALT="Figure 1"> View larger version (39K): org.highwire.dtl.DTLVardef@161b6b1org.highwire.dtl.DTLVardef@214d55org.highwire.dtl.DTLVardef@5c3ee8org.highwire.dtl.DTLVardef@263227_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Rios, M. S., Ripoll-Cladellas, A., Omidi, F., Ballouz, S., Alquicira-Hernandez, J., Oelen, R., Franke, L., van der Wijst, M. G. P., Powell, J. E., Mele, M.. 2024-11-08. Single-cell atlas of the human immune system reveals sex-specific dynamics of immunosenescence. https://doi.org/10.1101/2024.11.06.622096

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