Characterizing the effect of impeller design in plant cell fermentations using CFD modeling

Abstract

Cultivation of plant cell cultures in conventional bioreactors designed for microbial cells often results in the decrease of biomass productivity as compared to that in shake flasks, presumably due to the imbalance between the mass transfer requirements and compromise with cell viability. Hit and trial methods are usually adapted to achieve high biomass productivity in the bioreactor. In this study, a rational approach has been developed to choose a suitable impeller for Viola odorata cell suspension culture using computational fluid dynamics (CFD). A two-phase CFD model was employed to characterize the non-Newtonian fluid dynamics in a stirred tank reactor using different impeller designs, a setric, Rushton and marine impeller. The simulations were performed adopting Euler-Euler approach for the two-phase flow and dispersed{kappa} - {varepsilon} turbulence model. The numerical model was validated with good agreement with experimental determination of volumetric mass transfer coefficient. The impact of impeller design was then investigated on critical process parameters like mixing, oxygen mass transfer and shear. The developed CFD model demonstrated that setric impeller is a suitable choice for V. odorata cell cultivation offering low-shear environment at equivalent velocity magnitudes at reactor bottom with higher cell-lift capabilities which is preferable in high cell-density plant cell cultivations.