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bioRxiv · 10.1101/2024.11.04.621811

Spatiotemporal transcriptomic niches of complement pathway and serine protease inhibitor activation in aging and infection

Abstract

Aging is a multifactorial and complex physiological process, affecting every organ with characteristic manifestations. Understanding the molecular mechanisms that drive aging processes is crucial to targeting age-related disorders. Recent reports suggest that severe post-infection syndromes can partially accelerate aging. However, the underlying gene-encoded regulatory interplay, whether being shared or distinct between aging and infection biology are poorly understood. Here, we employed spatial transcriptomics to establish a multi-organ atlas (brain, heart, kidney, liver, lung, and spleen) across the mouse lifespan (4, 17, and 26 months). Dissecting high-quality fresh-frozen tissue samples at unbiased molecular resolution, we found both organ-specific and cross-organ gene dysregulation upon aging. We identified age-related trajectories in gene expression and cell state, some only detectable within their spatial context, and provide validation at subcellular resolution. The most prominent effect was organ-wide immune system activation with spatially variable severity. We therefore evaluated how aging mimics the expression signatures observed in systemic infection, using spatial transcriptomics slices from young mice infected with Plasmodium berghei ANKA. While on the gene level the effect sizes caused by the infection outweighed those of aging, we reveal a shared activation of the early complement pathway (C4b) and serine protease inhibitors (Serpin gene family) within by phenotype distinct spatial niches. We show that this common RNA signature is driven by tissue-specific cell types and eventually affects protein levels in the aged brain, rendering them a target for future mechanistic and drug discovery studies. Taken together, our study provides a coherent in-depth and cross-organ transcriptomics atlas to systematically study aging and infection in the mouse at spatiotemporal resolution. Key highlightsO_LILarge-scale and high-resolution atlas of spatial transcriptomics from six organs to study aging and systemic infection across two mouse cohorts. C_LIO_LIStrong transcriptional alterations found in distinct organ-specific niches for aging and acute malaria, with organ- and cell type-associated immune responses. C_LIO_LIDysregulation of early complement proteases (C4b) and serine protease inhibitors (Serpina3n) as common theme across central nervous system and peripheral organs. C_LI

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BibTeXRIS

Kern, F., Wagner, V., Wahl, V., Ludwig, N., Graf, S., Amand, J., Foltz, A., Atkins, M., Kedir, B., Hadjilaou, A., Flotho, M., Grandke, F., Wartenberg, P., Hollenhorst, M. I., Lotter, H., Mueller, R., Boehm, U., Hahn, O., Jacobs, T., Krasteva-Christ, G., Wyss-Coray, T., Keller, A.. 2024-11-04. Spatiotemporal transcriptomic niches of complement pathway and serine protease inhibitor activation in aging and infection. https://doi.org/10.1101/2024.11.04.621811

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