Evaluating the Biocontrol Potential of Pythium oligandrum and Serratia proteamaculans in Controlling Phytophthora plurivora in European beech (Fagus sylvatica)

Abstract

In recent years, the plant pathogen Phytophthora plurivora has caused severe damage to beech forests in many European countries, including Sweden. In many affected areas, few protective measures are in place due to fears about potential negative impacts on the ecosystem or public health. The research presented in this article assesses the biocontrol potential of the oomycete Pythium oligandrum and the bacterium Serratia proteamaculans against P. plurivora, with experiments performed under both in vitro and greenhouse conditions, which could represent a step forward in developing a safe treatment for European beech forests. The in vitro results revealed that P. oligandrum and S. proteamaculans significantly inhibited pathogen growth and stimulated a shift in the hyphal growth pattern towards shorter, branched hyphae with many hyphal swellings and thickened cell walls. The experiments conducted in greenhouses showed that treating three-month-old beech seedlings with P. oligandrum and S. proteamaculans counteracts the P. plurivora pathogen and reduces disease symptoms on the aerial and underground parts of the plant. GC-MS analysis detected the volatile organic compounds alpha-pinene, 2,5-dimethyl-pyrazine, and 3-methyl-1-butanol from S. proteamaculans; these compounds have the potential to inhibit pathogen growth. The disease suppression demonstrated by these biocontrol agents could thus be related to the synergistic effect of competition for nutrients with the secretion of hydrolytic enzymes and VOCs; moreover, induced systemic resistance may be triggered under greenhouse conditions. In conclusion, using P. oligandrum and S. proteamaculans could represent an environmentally friendly strategy for effectively controlling the disease caused by P. plurivora in beech.