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bioRxiv · 10.1101/2024.10.14.618231

The 3D point spread function can be autoencoded in latent space

Truedson, A.·Gras, K.·Elf, J.
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Abstract

The 3D point spread function of a fluorescence emitter in a living cell is often different from that of the z-stack of bead images typically used as a reference. Here we show that the location in z of a fluorescent emitter can be directly accessed in the latent space of an autoencoder trained on the sample images without z-reference data. The corresponding decoding network represents an accurate point spread function.

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Truedson, A., Gras, K., Elf, J.. 2024-10-15. The 3D point spread function can be autoencoded in latent space. https://doi.org/10.1101/2024.10.14.618231

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