eQTL in diseased colon tissue identifies novel target genes associated with IBD

Nishiyama, N. C.; Silverstein, S.; Darlington, K.; Kennedy Ng, M. M.; Clough, K. M.; Bauer, M.; Beasley, C.; Bharadwaj, A.; Ganesan, R.; Kapadia, M. R.; Lau, G.; Lian, G.; Rahbar, R.; Sadiq, T. S.; Schaner, M. R.; Stem, J.; Friton, J.; Faubion, W. A.; Sheikh, S. Z.; Furey, T. S.

doi:10.1101/2024.10.14.618229

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bioRxiv · 10.1101/2024.10.14.618229

eQTL in diseased colon tissue identifies novel target genes associated with IBD

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Abstract

Genome-wide association studies (GWAS) have identified over 300 loci associated with the inflammatory bowel diseases (IBD), but putative causal genes for most are unknown. We conducted the largest disease-focused expression quantitative trait loci (eQTL) analysis using colon tissue from 252 IBD patients to determine genetic effects on gene expression and potential contribution to IBD. Combined with two non-IBD colon eQTL studies, we identified 194 potential target genes for 108 GWAS loci. eQTL in IBD tissue were enriched for IBD GWAS loci colocalizations, provided novel evidence for IBD-associated genes such as ABO and TNFRSF14, and identified additional target genes compared to non-IBD tissue eQTL. IBD-associated eQTL unique to diseased tissue had distinct regulatory and functional characteristics with increased effect sizes. Together, these highlight the importance of eQTL studies in diseased tissue for understanding functional consequences of genetic variants, and elucidating molecular mechanisms and regulation of key genes involved in IBD.

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Nishiyama, N. C., Silverstein, S., Darlington, K., Kennedy Ng, M. M., Clough, K. M., Bauer, M., Beasley, C., Bharadwaj, A., Ganesan, R., Kapadia, M. R., Lau, G., Lian, G., Rahbar, R., Sadiq, T. S., Schaner, M. R., Stem, J., Friton, J., Faubion, W. A., Sheikh, S. Z., Furey, T. S.. 2024-10-17. eQTL in diseased colon tissue identifies novel target genes associated with IBD. https://doi.org/10.1101/2024.10.14.618229

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