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bioRxiv · 10.1101/2024.10.14.617924

Structure of the turnip yellows virus particles. How is the readthrough domain exposed on the capsid surface?

Lai-Kee-Him, J.·Trapani, S.·Boissinot, S.·Reinbold, C.·Fallet, C.·Ancelin, A.·Lecorre, F.·Hoh, F.·Ziegler-Graff, V.·Brault, V.·Bron, P.
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Abstract

Turnip yellows virus (TuYV) is a plant virus infecting important crops such as oilseed rape. TuYV is phloem-restricted and transmitted by aphids. The capsid contains two subunit types: the major capsid protein (CP) and a minor component (RTP*) which arises from the C-terminal cleavage of a readthrough product (RTP). RTP* contains the CP sequence fused with a structured domain, denoted NRTD, which is a key determinant of virus transmission. Though both CP and RTP* are involved in virus movement and aphid transmission, how RTP* is incorporated into the capsid is poorly understood. We present here the structural characterisation, by immunogold labelling and 3D cryo-EM, of the wild-type TuYV and a mutant whose capsid contains the CP only. We show that incorporation of RTP* does not impair the capsid structure, and the NRTD does not adopt well-defined positions at the capsid surface. The number of incorporated RTP*s suggests a random insertion.

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Lai-Kee-Him, J., Trapani, S., Boissinot, S., Reinbold, C., Fallet, C., Ancelin, A., Lecorre, F., Hoh, F., Ziegler-Graff, V., Brault, V., Bron, P.. 2024-10-14. Structure of the turnip yellows virus particles. How is the readthrough domain exposed on the capsid surface?. https://doi.org/10.1101/2024.10.14.617924

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