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bioRxiv · 10.1101/2024.10.14.617543

Light-guided actin polymerization drives directed motility in protocells

Abstract

Motility is a hallmark of lifes dynamic processes, enabling cells to actively chase prey, repair wounds, and shape organs. Recreating these intricate behaviors using well-defined molecules remains a major challenge at the intersection of biology, physics, and molecular engineering. Although the polymerization force of the actin cytoskeleton is characterized as a primary driver of cell motility, building a minimal platform to test this process in protocellular systems has proven elusive. The difficulty lies in the daunting task of distilling key components from motile cells and integrating them into model membranes in a physiologically relevant manner. To address this, we developed a method to optically control actin polymerization with high spatiotemporal precision within cell-mimetic lipid vesicles known as giant unilamellar vesicles (GUVs). Within these active protocells, the reorganization of actin networks triggered outward membrane extensions as well as the unidirectional movement of GUVs at speeds of up to 0.43 {micro}m/min, within the range of adherent mammalian cells. Notably, our findings reveal the requirements of both branched and linear actin networks for efficient membrane protrusions. This approach offers a powerful platform for unraveling the intricacies of cell migration, designing synthetic cells with active morphodynamics, and advancing bioengineering applications, such as self-propelled delivery systems and autonomous tissue-like materials.

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BibTeXRIS

Matsubayashi, H. T., Razavi, S., Rock, T. W., Nakajima, D., Nakamura, H., Kramer, D. A., Matsuura, T., Chen, B., Murata, S., Nomura, S.-i. M., Inoue, T.. 2024-10-15. Light-guided actin polymerization drives directed motility in protocells. https://doi.org/10.1101/2024.10.14.617543

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