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bioRxiv · 10.1101/2024.10.11.617934

LC-MS system for automatically collecting time-resolved metabolomics data of cultured cells

Chan, C. C. Y.·Groves, R. A.·Lewis, I. A.
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Abstract

Temporal metabolic dynamics are a critical, but difficult to study aspect of metabolism. To address this, we developed a liquid chromatography-mass spectrometry (LC-MS) system, temporal uptake and nutritional analysis (TUNA), to automatically collect time-resolved metabolomics data of cultured cells. TUNA enables sub-minute sequential sampling, has broad metabolite coverage, supports robust metabolite identification, can monitor over 72 conditions in parallel, and can be implemented in most LC-MS laboratories. We used TUNA to monitor temporal dynamics of uropathogens (Escherichia coli and Proteus mirabilis) and identify novel metabolic phenotypes that cannot be captured from a single time point.

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Chan, C. C. Y., Groves, R. A., Lewis, I. A.. 2024-10-12. LC-MS system for automatically collecting time-resolved metabolomics data of cultured cells. https://doi.org/10.1101/2024.10.11.617934

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