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bioRxiv · 10.1101/2024.10.05.616825

Computational Insights into the Allosteric Behavior of Mini-Insulin in Receptor Binding Driven by C-Peptide Mobility

AYAN, E.
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Abstract

Background/aimThe production of recombinant insulin remains challenging, particularly in enhancing refolding efficiency and bioactivity. Mini-proinsulin analogs, which involve reducing the length of the C-peptide, offer potential improvements in insulin production. This study aims to evaluate mini-proinsulin analogs design and receptor binding dynamics to optimize recombinant insulin production in E. coli. Materials and methodsMini-proinsulin analogs were engineered by replacing the 33-residue C-peptide with a pentapeptide sequence to improve refolding. The three-dimensional structure of mini-proinsulin was predicted using AlphaFold and performed docking analysis of mini-proinsulin analogs to the insulin receptor using AutoDock Tools, with comparisons made to previously available NMR-determined analog and the native insulin-insulin receptor complex. Normal Mode Analyses (GNM and ANM) were performed in detail to assess binding dynamics. ResultsIn silico analyses revealed that mini-proinsulin analogs closely replicate the structural features of native insulin and display receptor binding dynamics similar to native insulin, though they follow distinct receptor interaction paths. ConclusionAll analysis suggests that C-peptide mobility may contribute to the allosteric behavior observed in mini-proinsulin analogs during receptor interaction.

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AYAN, E.. 2024-10-06. Computational Insights into the Allosteric Behavior of Mini-Insulin in Receptor Binding Driven by C-Peptide Mobility. https://doi.org/10.1101/2024.10.05.616825

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