Ribozyme-based biosensor for imaging gene expression in plants

Liu, Y.; Rajput, R.; Islam, T.; Valle, I. D.; Yao, T.; Agarwal, R.; Boone, B. A.; Eckert, C. A.; Abraham, P. E.; Chen, J.-G.; Tuskan, G. A.; Yang, X.

doi:10.1101/2024.09.30.615876

bioRxiv · 10.1101/2024.09.30.615876

Ribozyme-based biosensor for imaging gene expression in plants

Liu, Y.·Rajput, R.·Islam, T.·Valle, I. D.·Yao, T.·Agarwal, R.·Boone, B. A.·Eckert, C. A.·Abraham, P. E.·Chen, J.-G.·Tuskan, G. A.·Yang, X.

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Abstract

Detection of gene expression in plants is critical for understanding the molecular basis of complex plant biosystems and plant responses to environmental stresses. Here, we report the development of a split ribozyme-based biosensor that enables in vivo visualization of gene expression in plants. We demonstrated the utility of this biosensor in transient expression experiments (i.e., leaf infiltration in Nicotiana benthamiana) to detect RNAs derived from transgenes and tobacco rattle virus, respectively. Furthermore, we successfully engineered a split ribozyme-based biosensor in Arabidopsis thaliana for in vivo visualization of endogenous gene expression at the cellular level. In addition, we developed a platform for easy incorporation of different reporters into the RNA biosensor.

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Liu, Y., Rajput, R., Islam, T., Valle, I. D., Yao, T., Agarwal, R., Boone, B. A., Eckert, C. A., Abraham, P. E., Chen, J.-G., Tuskan, G. A., Yang, X.. 2024-09-30. Ribozyme-based biosensor for imaging gene expression in plants. https://doi.org/10.1101/2024.09.30.615876

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