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bioRxiv · 10.1101/2024.09.26.614333

Protocol for simultaneous evaluation of neuronal activity and neurotransmitter release following chronic amyloid-beta oligomer injections into the hippocampus

Herve, V.·Bonenfant, L.·Amyot, M.·Balafrej, R.·Ali, O. B. K.·Benali, H.·Brouillette, J.
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Abstract

In Alzheimers disease, there is an imbalance in neurotransmitter release and altered neuronal activation. We present a novel approach to analyze neuronal activity by combining local field potential (LFP) recording with microdialysis within the same animal. This method measures glutamate and GABA levels following chronic hippocampal amyloid-beta oligomer (A{beta}o) injections in rats. We outline the design of our electrode and canula, the surgical procedure, and the steps for LFP recording, interstitial fluid collection, and A{beta}o injections simultaneously in living animal. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=182 HEIGHT=200 SRC="FIGDIR/small/614333v2_ufig1.gif" ALT="Figure 1"> View larger version (37K): org.highwire.dtl.DTLVardef@155f3c0org.highwire.dtl.DTLVardef@1f60289org.highwire.dtl.DTLVardef@207ba2org.highwire.dtl.DTLVardef@94eeb3_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Herve, V., Bonenfant, L., Amyot, M., Balafrej, R., Ali, O. B. K., Benali, H., Brouillette, J.. 2024-09-27. Protocol for simultaneous evaluation of neuronal activity and neurotransmitter release following chronic amyloid-beta oligomer injections into the hippocampus. https://doi.org/10.1101/2024.09.26.614333

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