A minor impact of VGLUT1 expression level on quantal size revealed through the characterization of VGLUT1mEos2 knock-down new mouse model

Zhang, X. M.; Francois, U.; Angelo, M. F.; Claverol, S.; Mondin, M.; Martin, C.; Deshors, M.; Humeau, Y.; Lipstein, N.; Herzog, E.

doi:10.1101/2024.09.23.614439

bioRxiv · 10.1101/2024.09.23.614439

A minor impact of VGLUT1 expression level on quantal size revealed through the characterization of VGLUT1mEos2 knock-down new mouse model

Zhang, X. M.·Francois, U.·Angelo, M. F.·Claverol, S.·Mondin, M.·Martin, C.·Deshors, M.·Humeau, Y.·Lipstein, N.·Herzog, E.

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Abstract

Synaptic vesicles (SVs) are small organelles secreting neurotransmitters at synapses. By fusing a photoactivated fluorescent protein to VGLUT1, we generated a VGLUT1mEos2 knock-in mouse. VGLUT1mEos2 knock-in mice are viable and healthy, but exhibit a severe reduction in VGLUT1 expression levels. Using VGLUT1mEos2 expressing neurons, we established paradigms to trace individual SV mobility at the single-molecule level or via massive photoconversion. Hippocampal neurons with significantly diminished VGLUT1 expression maintain unaltered miniature glutamate release characteristics in terms of quantal size and frequency. We demonstrate that VGLUT1 expression level are not correlated in a linear fashion with the vesicular glutamate content. In conclusion, the VGLUT1mEos2 mouse line serves as a powerful tool for exploring SV mobility properties and elucidating the contributions of VGLUT1 to excitatory neurotransmission and cognitive processes.

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Zhang, X. M., Francois, U., Angelo, M. F., Claverol, S., Mondin, M., Martin, C., Deshors, M., Humeau, Y., Lipstein, N., Herzog, E.. 2024-09-24. A minor impact of VGLUT1 expression level on quantal size revealed through the characterization of VGLUT1mEos2 knock-down new mouse model. https://doi.org/10.1101/2024.09.23.614439

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