Sex-specific effects of early life unpredictability on hippocampal and amygdala responses to novelty in adolescents

Davis, E. P.; Leonard, B.; Jirsaraie, R.; Keator, D.; Small, S.; Sandman, C. A.; Risbrough, V.; Stern, H. S.; Glynn, L.; Yassa, M. A.; Baram, T. Z.; Rasmussen, J.

doi:10.1101/2024.09.20.614130

bioRxiv · 10.1101/2024.09.20.614130

Sex-specific effects of early life unpredictability on hippocampal and amygdala responses to novelty in adolescents

Davis, E. P.·Leonard, B.·Jirsaraie, R.·Keator, D.·Small, S.·Sandman, C. A.·Risbrough, V.·Stern, H. S.·Glynn, L.·Yassa, M. A.·Baram, T. Z.·Rasmussen, J.

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Abstract

BackgroundUnpredictable childhood experiences are an understudied form of early life adversity that impacts neurodevelopment in a sex-specific manner. The neurobiological processes by which exposure to early-life unpredictability impacts development and vulnerability to psychopathology remain poorly understood. The present study investigates the sex-specific consequences of early-life unpredictability on the limbic network, focusing on the hippocampus and the amygdala. MethodsParticipants included 150 youth (54% female). Early life unpredictability was assessed using the Questionnaire of Unpredictability in Childhood (QUIC). Participants engaged in a task-fMRI scan between the ages of 8 and 17 (223 total observations) measuring BOLD responses to novel and familiar scenes. ResultsExposure to early-life unpredictability associated with BOLD contrast (novel vs. familiar) in a sex-specific manner. For males, but not females, higher QUIC scores were associated with lower BOLD activation in response to novel vs. familiar stimuli in the hippocampal head and amygdala. Secondary psychophysiological interaction (PPI) analyses revealed complementary sex-specific associations between QUIC and condition-specific functional connectivity between the right and left amygdala, as well as between the right amygdala and hippocampus bilaterally. ConclusionExposure to unpredictability in early life has persistent implications for the functional operations of limbic circuits. Importantly, consistent with emerging experimental animal and human studies, the consequences of early life unpredictability differ for males and females. Further, impacts of early-life unpredictability were independent of other risk factors including lower household income and negative life events, indicating distinct consequences of early-life unpredictability over and above more commonly studied types of early life adversity.

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Davis, E. P., Leonard, B., Jirsaraie, R., Keator, D., Small, S., Sandman, C. A., Risbrough, V., Stern, H. S., Glynn, L., Yassa, M. A., Baram, T. Z., Rasmussen, J.. 2024-09-23. Sex-specific effects of early life unpredictability on hippocampal and amygdala responses to novelty in adolescents. https://doi.org/10.1101/2024.09.20.614130

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