TYPE THREE SECRETION SYSTEM-INDUCED MECHANOPORATION OF VACUOLAR MEMBRANES

SWISTAK, L.; Albert, M.; Valenzuela, C.; Gokerkucuk, E. B.; Bontems, F.; Gazi, A. D.; Tachon, S.; Sartori-Rupp, A.; Lesser, C. F.; Paul-Gilloteaux, P.; Tinevez, J.-Y.; Vos, M.; Enninga, J.

doi:10.1101/2024.09.19.613840

bioRxiv · 10.1101/2024.09.19.613840

TYPE THREE SECRETION SYSTEM-INDUCED MECHANOPORATION OF VACUOLAR MEMBRANES

SWISTAK, L.·Albert, M.·Valenzuela, C.·Gokerkucuk, E. B.·Bontems, F.·Gazi, A. D.·Tachon, S.·Sartori-Rupp, A.·Lesser, C. F.·Paul-Gilloteaux, P.·Tinevez, J.-Y.·Vos, M.·Enninga, J.

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Abstract

SO_SCPLOWUMMARYC_SCPLOWEndomembrane breaching is a crucial strategy employed by intracellular pathogens enclosed within vacuoles to access the nutrient-rich cytosol for intracellular replication. While bacteria use various mechanisms to compromise host membranes, the specific processes and factors involved are often unknown. Shigella flexneri, a major human pathogen, accesses the cytosol relying on the Type Three Secretion System (T3SS) and secreted effectors. Using in-cell correlative light and electron microscopy, we tracked the sequential steps of Shigella host cell entry. Moreover, we captured the T3SS, which projects a needle from the bacterial surface, in the process of puncturing holes in the vacuolar membrane. This initial puncture ensures disruption of the vacuolar membrane. Together this introduces the concept of mechanoporation via a bacterial secretion system as a crucial process for bacterial pathogen-induced membrane damage. GO_SCPLOWRAPHICALC_SCPLOW AO_SCPLOWBSTRACTC_SCPLOW O_FIG O_LINKSMALLFIG WIDTH=199 HEIGHT=200 SRC="FIGDIR/small/613840v1_ufig1.gif" ALT="Figure 1"> View larger version (54K): org.highwire.dtl.DTLVardef@1669028org.highwire.dtl.DTLVardef@8259cborg.highwire.dtl.DTLVardef@1d522caorg.highwire.dtl.DTLVardef@1df0f2d_HPS_FORMAT_FIGEXP M_FIG C_FIG

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SWISTAK, L., Albert, M., Valenzuela, C., Gokerkucuk, E. B., Bontems, F., Gazi, A. D., Tachon, S., Sartori-Rupp, A., Lesser, C. F., Paul-Gilloteaux, P., Tinevez, J.-Y., Vos, M., Enninga, J.. 2024-09-19. TYPE THREE SECRETION SYSTEM-INDUCED MECHANOPORATION OF VACUOLAR MEMBRANES. https://doi.org/10.1101/2024.09.19.613840

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