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bioRxiv · 10.1101/2024.09.17.613460

A high-resolution microscopy system for biological studies of cold-adapted species under physiological conditions

Marty, A.-P. M.·Ward, E. N.·Lamb, J. R.·van Tartwijk, F. W.·Peck, L. S.·Clark, M. S.·Kaminski, C. F.
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Abstract

The fundamental processes governing life are sensitively dependent on temperature. Whilst much is known about the constraints on how proteins operate at 37{degrees}C, little knowledge exists about how biological function is maintained sub-zero temperature conditions, where proteins are less stable and oxidative damage is high. However, almost 90% of habitable environments on Earth are permanently below 5{degrees}C (i.e. the deep sea and polar regions). This means that we do not understand how a large and diverse proportion of the global biome functions. To address this question at the cellular level, tools are required for imaging biological systems at high resolution under physiological conditions. This poses severe technical challenges that cannot be addressed with traditional optical microscopy techniques. High-resolution imaging objectives require short working distances and the use of immersion media, which lead to rapid heat transfer from the microscope to the sample. This affects the viability of live specimens and the interpretability of the results when the sample function optimally at low temperatures. Condensation and temperature-induced shrinking of components pose further challenges, reducing image resolution and contrast. Here, we address these issues and provide a method for high-fidelity imaging of live biological samples at temperatures of around, or below, 0{degrees}C. Our method is compatible with different microscopy modalities, including super-resolution imaging. It relies on hardware additions to traditional microscopy systems that can be straightforwardly implemented, namely, a cooling collar, 10% ethanol as an immersion medium, and nitrogen flow to mitigate condensation. We demonstrate the method in live cell cultures derived from Antarctic fish species and highlight the need to maintain physiological conditions for these fragile biological samples. Future applications are diverse and include evolutionary biology and the study of cold-adapted organisms, as well as cellular biophysics and several applications in biotechnology.

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Marty, A.-P. M., Ward, E. N., Lamb, J. R., van Tartwijk, F. W., Peck, L. S., Clark, M. S., Kaminski, C. F.. 2024-09-20. A high-resolution microscopy system for biological studies of cold-adapted species under physiological conditions. https://doi.org/10.1101/2024.09.17.613460

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