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bioRxiv · 10.1101/2024.09.12.612736

CRAK-Velo: Chromatin Accessibility Kinetics integration improves RNA Velocity estimation

El Kazwini, N.·Gao, M.·Boudjelthia, I. K.·Cai, F.·Huang, Y.·Sanguinetti, G.
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Abstract

RNA velocity has recently emerged as a key tool in the analysis of single-cell transcriptomic data, yet connecting RNA velocity analyses to underlying regulatory processes has proved challenging. Here we propose CRAK-Velo, a semi-mechanistic model which integrates chromatin accessibility data in the estimation of RNA velocities. CRAK-Velo provides biologically consistent estimates of developmental flows and enables accurate cell-type deconvolution, while additionally shining light on regulatory processes at the level of interactions between genes and chromatin regions.

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El Kazwini, N., Gao, M., Boudjelthia, I. K., Cai, F., Huang, Y., Sanguinetti, G.. 2024-09-17. CRAK-Velo: Chromatin Accessibility Kinetics integration improves RNA Velocity estimation. https://doi.org/10.1101/2024.09.12.612736

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