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bioRxiv · 10.1101/2024.09.10.612264

Rapid production of modified cattle and sheep from spermatid-like stem cells

Yang, L.·Di, A.·Song, L.·Liu, X.·Wu, D.·Wang, S.·Hao, Z.·Bu, L.·Bai, C.·Su, G.·Wei, Z.·Zhang, L.·Liu, Z.·Gao, S.·Li, G.
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Abstract

Genome-modified ruminants, such as cattle and sheep, are important germplasm for agricultural applications. Haploid androgenetic stem cells (haSCs) can support the full-term development of embryos upon intracytoplasmic haSCs injection (iCHI) into intact oocytes. Thus, haSCs are invaluable resources for studying economic traits and significantly impact livestock breeding; however, ruminant haSCs have yet to be obtained. Here, we report the derivation of cattle and sheep haSCs using a recombinant FACE medium. These cells display characteristics of formative-state pluripotency and can differentiate into three germ layers, both in vitro and in vivo. Interestingly, the ectopic expression of protamine in haSCs converts their nuclei into a spermatid-like structure and synergistic enhancement of full-term development of iCHI embryos, named as Pro-iCHI approach. Furthermore, gene-modified cattle and sheep can be efficiently produced through the combinatorial use of the CRISPR and Pro-iCHI approach. Overall, Pro-iCHI provides an easy-to-manipulate agrobiotechnology tool for rapidly producing genetically modified livestock.

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Yang, L., Di, A., Song, L., Liu, X., Wu, D., Wang, S., Hao, Z., Bu, L., Bai, C., Su, G., Wei, Z., Zhang, L., Liu, Z., Gao, S., Li, G.. 2024-09-14. Rapid production of modified cattle and sheep from spermatid-like stem cells. https://doi.org/10.1101/2024.09.10.612264

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