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bioRxiv · 10.1101/2024.09.04.611284

Proteomic Investigation of Neurotrophic trans-Banglene Reveals Potential Link to Iron Homeostasis

Gunawardana, P. W.·Gohil, K.·Moon, K.-M.·Foster, L. J.·Williams, F. J.
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Abstract

In an effort to gain insight into cellular systems impacted by neurotrophic trans-banglene (t-BG), global proteomic profiling and Western blot analyses were employed. Expression level changes in response to t-BG treatment were compared to those observed with nerve growth factor (NGF), a natural neurotrophic protein and functional analog to t-BG. Findings from these studies did not point to direct interception of NGF/TrkA signaling by t-BG. Instead, significant alterations in iron-binding and iron-regulating proteins were observed. Intracellular iron measurements by FerroOrange indicate lower ferrous (Fe2+) iron levels in t-BG treated cells but not in NGF treated cells. These results highlight a potential connection between iron regulation and neurotrophic activity.

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Gunawardana, P. W., Gohil, K., Moon, K.-M., Foster, L. J., Williams, F. J.. 2024-09-04. Proteomic Investigation of Neurotrophic trans-Banglene Reveals Potential Link to Iron Homeostasis. https://doi.org/10.1101/2024.09.04.611284

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