Limited microglial metabolic improvement with time-restricted feeding in diet-induced obesity

Jiao, H.; Jermei, J.; Liang, X.; Correa-da-Silva, F.; Dorscheidt, M.; Rumanova, V. S.; Poormoghadam, D.; Foppen, E.; Korpel, N.; Stenvers, D. J.; Arias, A. P.; Liu, T.; Zhi, Z.; Kalsbeek, A.; Yi, C.-X.

doi:10.1101/2024.09.04.611250

bioRxiv · 10.1101/2024.09.04.611250

Limited microglial metabolic improvement with time-restricted feeding in diet-induced obesity

Jiao, H.·Jermei, J.·Liang, X.·Correa-da-Silva, F.·Dorscheidt, M.·Rumanova, V. S.·Poormoghadam, D.·Foppen, E.·Korpel, N.·Stenvers, D. J.·Arias, A. P.·Liu, T.·Zhi, Z.·Kalsbeek, A.·Yi, C.-X.

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Abstract

Time-restricted eating has shown promise for improving metabolic health in obese humans via incompletely resolved mechanisms. In this study, we investigated how time-restricted feeding (TRF) at different times of the day affects microglial immunometabolism using Wistar rats. We found that in high-fat diet (HFD)-fed obese rats, TRF during the active phase reduced fat mass, altered rhythmicity of the microglial transcriptome, and prevented an increase in hypothalamic microglia. These effects were dampened or absent with TRF during the resting phase. However, a HFD-induced microglial immunometabolic phenotype, characterized by reduced electron transport chain and increased lipid metabolism gene expression, and metabolic inflexibility, was not reversed by TRF in either the active or resting phase, indicating that reprogrammed microglial metabolism in obesity is a persistent cellular functional change that requires further study.

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Jiao, H., Jermei, J., Liang, X., Correa-da-Silva, F., Dorscheidt, M., Rumanova, V. S., Poormoghadam, D., Foppen, E., Korpel, N., Stenvers, D. J., Arias, A. P., Liu, T., Zhi, Z., Kalsbeek, A., Yi, C.-X.. 2024-09-06. Limited microglial metabolic improvement with time-restricted feeding in diet-induced obesity. https://doi.org/10.1101/2024.09.04.611250

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