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bioRxiv · 10.1101/2024.09.03.611064

Direct Nuclear Delivery of Proteins on Living Plant via Partial Enzymatic Cell Wall Digestion

Gu, Q.·Ming, N.·Aizezi, Y.·Wei, X.·Yuan, Y.·Esquivel, B.·Wang, Z.-Y.
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Abstract

While many variations of protein delivery methods have been described, it can still be difficult or inefficient to introduce exogenous proteins into plants. A major barrier to progress is the cell wall which is primarily composed of polysaccharides and thus only permeable to small molecules. Here, we report a partial enzymatic cell wall digestion-mediated uptake method that efficiently delivers protein into the nucleus of plant cells. Such a method allowed efficient nuclear delivery of GFP proteins into Arabidopsis root cells throughout all cell layers. This study establishes that a partial enzymatic cell wall degradation could enable a myriad of plant biotechnology applications that rely on functional protein delivery into walled plant cells.

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Gu, Q., Ming, N., Aizezi, Y., Wei, X., Yuan, Y., Esquivel, B., Wang, Z.-Y.. 2024-09-07. Direct Nuclear Delivery of Proteins on Living Plant via Partial Enzymatic Cell Wall Digestion. https://doi.org/10.1101/2024.09.03.611064

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