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bioRxiv · 10.1101/2024.09.01.610697

Improved method for detecting protein-protein interactions using proximity ligation assay

Tower, Z.·Chang, H.
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Abstract

Proximity ligation assay has been widely used to detect protein-protein interaction in cells and tissues. While with great sensitivity, its specificity was often neglected. Here, we report the existence of varying levels of false positives observed with this assay and provide suggestions to minimize false positives for more accurate detection of protein-protein interactions, especially for membrane proteins.

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Tower, Z., Chang, H.. 2024-09-02. Improved method for detecting protein-protein interactions using proximity ligation assay. https://doi.org/10.1101/2024.09.01.610697

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