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bioRxiv · 10.1101/2024.08.28.610092

TGF-β signaling promotes the expression of proviral circular RNA ciTRAN in HIV-1 infection

Choudhary, A.·Malick, K.·Dalavi, R.·Chande, A.
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Abstract

Circular RNA (circRNA) expression is widespread in immune cells infected by HIV-1, but the crosstalk between circRNA expression and various cellular signaling pathways remains unclear. We report that HIV-1 Vpr triggers TGF-{beta} signaling, which we linked to the increased expression of ciTRAN, a proviral circRNA encoded by SMARCA5. Consistent with this finding, we observed that the essential intracellular TGF-{beta} receptor signaling component SMAD2/3 was recruited to the SMARCA5 promoter in a Vpr-dependent manner. SMARCA5 promoter analysis and functional assays further revealed that the SAMD2/3 binding motif is crucial for ciTRAN upregulation. In response to treatment with DNA-damaging agents or the exogenous addition of recombinant TGF-{beta}, the TGF-{beta}-SMAD axis upregulated the expression of ciTRAN as well as the parental SMARCA5 mRNA. Finally, pharmacological targeting of TGF-{beta} signaling or genetic ablation of TGFBR1 can reduce the ability of HIV-1 Vpr to induce the expression of ciTRAN and viral genes. These results offer crucial mechanistic insights into the regulation of ciTRAN expression by TGF-{beta} signaling and suggest a strategy to inhibit circRNA expression during infection by small molecules.

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Choudhary, A., Malick, K., Dalavi, R., Chande, A.. 2024-08-30. TGF-β signaling promotes the expression of proviral circular RNA ciTRAN in HIV-1 infection. https://doi.org/10.1101/2024.08.28.610092

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