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bioRxiv · 10.1101/2024.08.26.609762

Metadichol induces CD14 Glycoprotein Expression in Human Embryonic Stem Cells and Fibroblasts

Raghavan, P. R. E.
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Abstract

CD14, or cluster of differentiation 14, is a glycoprotein essential to the immune system and is found primarily on monocytes, macrophages, and other immune cells. Despite its importance, there are no examples in the literature of small compounds that can induce multifold expression of CD14 in human embryonic stem cells (hESCs) or fibroblasts. This study addresses this gap by exploring the potential of metadichol, a nanoemulsion of long-chain fatty alcohols, to induce CD14 expression in hESCs. Using quantitative real-time PCR (qRT{square}PCR) and Western blotting techniques, we showed that metadichol significantly upregulated CD14 expression by seventeen -fold in hESCs but downregulated it in fibroblasts. This novel finding indicates that metadichol can modulate CD14 expression in a cell type-specific manner, highlighting its potential for enhancing stem cell-based therapeutics and advancing our understanding of stem cell biology. The implications of these findings are substantial, suggesting new directions for research into the immune modulatory functions of hESCs and their potential applications in regenerative medicine. Our work highlights the potential of metadichol as a powerful tool for modulating CD14 expression in stem as well as somatic cells marking a significant step forward in the field of stem cell research and therapeutic development.

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Raghavan, P. R. E.. 2024-08-27. Metadichol induces CD14 Glycoprotein Expression in Human Embryonic Stem Cells and Fibroblasts. https://doi.org/10.1101/2024.08.26.609762

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