Cyclic Electromagnetic DNA Simulation (CEDS) influences the functions of Long Oligo-dsDNAs and Plasmid DNAs

Abstract

Hydrogen bonding magnetic resonance-based cyclic electromagnetic DNA simulation (CEDS) was proposed to play a role in regulating the function of target short oligo-dsDNA in the previous study, as the decagonal CEDS was found to induce the hybridization and conformational changes of target short oligo-dsDNA in a sequence-specific manner. This study applied dodecagonal CEDS on 24 bps oligo-dsDNAs and plasmid DNAs. CEDS can affect 24 bps oligo-dsDNAs intercalated by ethidium bromide (EtBr) or condensed by spermidine by stimulating them to partially remove EtBr or spermidine, respectively, depending on CEDS time. When the multiple cloning site of pBluescript was digested with eight restriction endonucleases (RE) under CEDS separately, all RE digests were enhanced depending on CEDS time compared to the negative and positive controls both in EtBr intercalated electrophoresis and HPLC analysis. In vitro RNA transcription from human VEGFA, vWF or elafin cDNA subcloned into pBluescript DNA was increased by CEDS using each promoter sequence depending on CEDS time compared to negative and positive controls. In addition, the productions of green fluorescent protein (GFP) and {beta}-galactosidase were enhanced by CEDS using each promoter sequence from pE-GFP-1 and pBluescript, respectively. In the survival assay using E. coli transfected with pBluescript containing ampicillin resistance gene, the number of surviving E. coli was increased by CEDS using a T3 promoter sequence compared to the control during two days of culture, while it was decreased by CEDS using a T7 promoter, non-specific 12A, 6(TA), or mutation-2 (GG18,19CC) T3 promoter sequence. Therefore, it is suggested that dodecagonal CEDS can target 24 bps oligo-dsDNAs and promoter sequences of plasmid DNAs in a sequence-specific manner and influence their functions.