Functional high capacity exosome-encapsulating bioinspired hydrogel promotes microvascularbed expansion in diabetic mice

Subhan, B. S.; Cortes Troncoso, J. F.; Katyal, P.; Meleties, M.; Mestvirishvili, T.; Arias, F.; Wang, A.; Ruggles, K.; Dolgalev, I.; Mita, P.; Montclare, J. K.; Rabbani, P. S.

doi:10.1101/2024.08.23.609402

bioRxiv · 10.1101/2024.08.23.609402

Functional high capacity exosome-encapsulating bioinspired hydrogel promotes microvascularbed expansion in diabetic mice

Subhan, B. S.·Cortes Troncoso, J. F.·Katyal, P.·Meleties, M.·Mestvirishvili, T.·Arias, F.·Wang, A.·Ruggles, K.·Dolgalev, I.·Mita, P.·Montclare, J. K.·Rabbani, P. S.

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Abstract

Chronic wounds present a significant clinical challenge due to impaired skin microvasculature, particularly in diabetes. The tissue engineering study introduces therapeutic Exo-Q, a unique thermoresponsive polymer hydrogel created with Q protein nanofibers and cultured human bone marrow multipotent stromal cell exosomes with consistent gene signature. Limited, local and topical application of Exo-Q hydrogel is feasible for maximum neovascularization during murine diabetic wound closure in a xenotransplantation model, as well as compatibility and vascular delivery in human skin in a xenograft model. Exo-Q hydrogel treatment significantly reduces diabetic wound closure time within ranges of non-diabetic wounds. This innovative, non-invasive tissue engineered therapeutic option offers a promising approach to addressing the complex pathologies of non-healing wounds.

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Subhan, B. S., Cortes Troncoso, J. F., Katyal, P., Meleties, M., Mestvirishvili, T., Arias, F., Wang, A., Ruggles, K., Dolgalev, I., Mita, P., Montclare, J. K., Rabbani, P. S.. 2024-08-26. Functional high capacity exosome-encapsulating bioinspired hydrogel promotes microvascularbed expansion in diabetic mice. https://doi.org/10.1101/2024.08.23.609402

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