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bioRxiv · 10.1101/2024.08.20.608123

Large scale comparative wheat phosphoproteome profiling pinpoints temperature-associated molecular signatures

Fonseca de Lima, C. F.·Zhu, T.·Van den Broeck, L.·Van De Cotte, B.·Locke, A. M.·Sozzani, R.·De Smet, I.
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Abstract

Elevated temperatures resulting from climate change adversely affect natural and crop ecosystems, necessitating the development of heat-tolerant crops. We established a framework to precisely identify wheat protein-phosphorylated sites associated with varying temperature sensitivities. We identified specific kinases primarily associated with a specific temperature, but our results also suggest a striking overlap between cold and heat signaling. We furthermore demonstrated that the phosphorylation state of a specific set of proteins creates a unique signature for heat stress tolerance. These findings can potentially aid in the identification of targets for breeding or genome editing to enhance sub/supra optimal temperature tolerance.

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Fonseca de Lima, C. F., Zhu, T., Van den Broeck, L., Van De Cotte, B., Locke, A. M., Sozzani, R., De Smet, I.. 2024-08-21. Large scale comparative wheat phosphoproteome profiling pinpoints temperature-associated molecular signatures. https://doi.org/10.1101/2024.08.20.608123

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