The consequences of mismatched buffers in spectral cell sorting

Dapaah, R. A. S.; Ferrer Font, L.; Shi, X.; Hall, C.; Thompson, S.; Catharina Costa, L.; Mage, P. L.; Tyznik, A. J.; Lundsten, K.; Walker, R. V.

doi:10.1101/2024.08.19.608560

bioRxiv · 10.1101/2024.08.19.608560

The consequences of mismatched buffers in spectral cell sorting

Dapaah, R. A. S.·Ferrer Font, L.·Shi, X.·Hall, C.·Thompson, S.·Catharina Costa, L.·Mage, P. L.·Tyznik, A. J.·Lundsten, K.·Walker, R. V.

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Abstract

Although spectral flow cytometry has become a ubiquitous tool for cell analysis, the use of spectral cytometry on cell sorters requires additional considerations arising from the unique requirements of sorting workflows. Here, we show that care should be taken when ascertaining the purity of a sort on a spectral cell sorter, as the mismatch of buffers used for initial sample suspension and the buffers used for sort collection can affect the unmixing of the data, potentially giving rise to erroneous purity check results.

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Dapaah, R. A. S., Ferrer Font, L., Shi, X., Hall, C., Thompson, S., Catharina Costa, L., Mage, P. L., Tyznik, A. J., Lundsten, K., Walker, R. V.. 2024-08-19. The consequences of mismatched buffers in spectral cell sorting. https://doi.org/10.1101/2024.08.19.608560

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