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bioRxiv · 10.1101/2024.08.10.607181

The eduWOSM: an advanced optical microscope for education

Carter, N. J.·Martin, D. S.·Molloy, J. E.·Cross, R. A.
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Abstract

To improve access to advanced optical microscopy in educational and resource-limited settings we have developed the eduWOSM (educational Warwick Open Source Microscope), an open hardware platform for transmitted-light and epifluorescence imaging in up to 4 colours, including single molecule imaging. EduWOSMs are robust, bright, compact, portable and ultra-stable. They are controlled entirely by open source hardware and software, with an option for remote control from a webpage. Here we describe the core eduWOSM technology and benchmark its performance using 3 example projects, single fluorophore tracking of tubulin heterodimers within gliding microtubules, 4D (deconvolution) imaging/tracking of chromosome motions in dividing human cells, and automated single particle tracking in vitro and in live cells with classification into subdiffusive, diffusive and superdiffusive motion.

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Carter, N. J., Martin, D. S., Molloy, J. E., Cross, R. A.. 2024-08-10. The eduWOSM: an advanced optical microscope for education. https://doi.org/10.1101/2024.08.10.607181

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