Rpt5 encodes a receptor-like protein that provides broad and effective net form net blotch (Pyrenophora teres f. teres) resistance in barley

Effertz, K.; Richards, J. K.; Clare, S. J.; del Castillo, M.; Sharma Poudel, R.; Li, M.; Zhang, J.; Moscou, M. J.; Friesen, T.; Brueggeman, R. S.

doi:10.1101/2024.08.09.607401

bioRxiv · 10.1101/2024.08.09.607401

Rpt5 encodes a receptor-like protein that provides broad and effective net form net blotch (Pyrenophora teres f. teres) resistance in barley

Effertz, K.·Richards, J. K.·Clare, S. J.·del Castillo, M.·Sharma Poudel, R.·Li, M.·Zhang, J.·Moscou, M. J.·Friesen, T.·Brueggeman, R. S.

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Abstract

The foliar disease net form net blotch (NFNB), caused by the necrotrophic fungal pathogen Pyrenophora teres f. teres (Ptt), causes significant yield and quality losses of barley worldwide. Dominant resistance conferred by the Resistance to Pyrenophora teres 5(Rpt5) gene from barley line CI5791 is the broadest and most effective resistance reported in this pathosystem. The Rpt5 locus was identified in multiple independent genetic studies utilizing diverse host populations and Ptt isolates, and harbors both dominant Rpt5 resistance and isolate-specific susceptibility genes/alleles that are dominant in the absence of Rpt5, designated susceptibility to Pyrenophora teres 1 (Spt1). Ptt virulence and avirulence effectors from diverse pathogen isolates genetically interact with the Rpt5/Spt1 locus, suggesting a complex locus with a function targeted by the evolution of a diversity of pathogen effectors. High-resolution mapping utilizing 1,920 recombinant gametes from a CI5791 x Tifang biparental population, identified 12 candidate genes in an [~]4.6 Mb delimited region in the cv Morex V3 genome assembly, but is 1.1 - 2.2 Mb in the pangenome assemblies, containing 5-12 genes. Analysis revealed a strong correlation between the CI5791 allele of a receptor-like protein (RLP), provisionally designated Rpt5 candidate gene 1, (Rcg1), and broad Rpt5-mediated resistance. Two independent transformants of the CI5791 Rcg1 allele in the susceptible cv Golden Promise background showed significantly increased resistance when challenged with Rpt5 avirulent Ptt isolates 6A, 15A, and 0-1 compared to the Golden Promise wildtype. Thus, Rpt5, encodes an RLP and is the first net blotch resistance gene cloned in barley. Significance StatementNet form net blotch (NFNB) causes substantial yield and quality losses of barley worldwide. Despite decades of ongoing research into genetic resistance to Pyrenophora teres f. teres, the causal agent of NFNB, no host resistance or susceptibility genes in this pathosystem had been previously identified. We present the identification and validation of Rpt5, which confers the broadest and most effective resistance against NFNB. To our knowledge Rpt5 is the first genetically defined resistance gene in a monocot to encode an RLP.

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Effertz, K., Richards, J. K., Clare, S. J., del Castillo, M., Sharma Poudel, R., Li, M., Zhang, J., Moscou, M. J., Friesen, T., Brueggeman, R. S.. 2024-08-10. Rpt5 encodes a receptor-like protein that provides broad and effective net form net blotch (Pyrenophora teres f. teres) resistance in barley. https://doi.org/10.1101/2024.08.09.607401

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