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bioRxiv · 10.1101/2024.08.09.606593

taxmyPHAGE: Automated taxonomy of dsDNA phage genomes at the genus and species

Millard, A. D.·Denise, R.·Lestido, M.·Thomas, M.·Webster, D.·Turner, D.·Sicheritz-Ponten, T.
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Abstract

BackgroundBacteriophages are now classified into genera and species based on genomic similarity. To identify new species and genera requires comparison against all phage genomes currently defined by the International Committee on the Taxonomy of Viruses. With increasing amounts of phage genomic data there is a clear need to fully automate the classification of phages into existing and new genera and species. Materials and MethodsWe created a MASH database for rapid searching of closely related phages, to then be compared with BLASTn to compute intergenomic similarity between bacteriophages (https://github.com/amillard/tax_myPHAGE/tree/main). ResultsTaxmyphage is provided as a conda repository and via a user-friendly website, that allows rapid classification of phages into existing taxa at the genus and species level. ConclusionsTaxmyphage allows the rapid and accurate classification of phage genomes into existing genera and species, at a scale that is compatible with existing sequencing output.

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Millard, A. D., Denise, R., Lestido, M., Thomas, M., Webster, D., Turner, D., Sicheritz-Ponten, T.. 2024-08-10. taxmyPHAGE: Automated taxonomy of dsDNA phage genomes at the genus and species. https://doi.org/10.1101/2024.08.09.606593

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