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bioRxiv · 10.1101/2024.08.05.606625

HIF1α controls steroidogenesis under acute hypoxic stress

Abstract

BackgroundHypoxia is a critical physiological and pathological condition known to influence various cellular processes, including steroidogenesis. While previous studies, including our own, have highlighted the regulatory effects of Hypoxia-Inducible Factor 1 (HIF1) on steroid production, the specific molecular mechanisms remain poorly understood. This study investigates the role of hypoxia and HIF1 in steroid biosynthesis across multiple experimental models during acute exposure to low oxygen levels. MethodsTo assess the extent to which acute hypoxia modulates steroidogenesis, we employed several approaches, including the Y1 adrenocortical cell line, an ex vivo adrenal gland explant model, and a conditional HIF1-deficient mouse line in the adrenal cortex. We focused on various regulatory patterns that may critically suppress steroidogenesis. ResultsIn Y1 cells and adrenal gland explants, hypoxia induced the upregulation of specific microRNAs, leading to the suppression of mRNA levels of key steroidogenic enzymes and reduced steroid hormone production. The hypoxia/HIF1-dependent induction of these microRNAs and the consequent modulation of steroid production were confirmed in vivo. Notably, using our conditional HIF1-deficient mouse line, we found that the increase in miRNA expression under hypoxic conditions is directly dependent on HIF1. Furthermore, the regulation of steroidogenic enzymes (e.g., StAR and Cyp11a1) and steroid production occurred at the level of protein translation, revealing an unexpected layer of control under hypoxic conditions in vivo. ConclusionsThese findings elucidate the molecular mechanisms underlying acute hypoxia-induced changes in steroid biosynthesis and may also be useful in developing new strategies for various steroid hormone pathologies.

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BibTeXRIS

Ariyeloye, S., Watts, D., Jaykar, M., Ermis, C., Krueger, A., Kaden, D., Stepien, B. K., Alexaki, V. I., Peitzsch, M., Bechmann, N., El-Armouche, A., Wielockx, B.. 2024-08-05. HIF1α controls steroidogenesis under acute hypoxic stress. https://doi.org/10.1101/2024.08.05.606625

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