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bioRxiv · 10.1101/2024.08.01.606198

Systems-level reconstruction of kinase phosphosignaling networks regulating endothelial barrier integrity using temporal data

Wei, L.·Mast, F. D.·Aitchison, J. D.·Kaushansky, A.
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Abstract

Phosphosignaling networks control cellular processes. We built kinase-mediated regulatory networks elicited by thrombin stimulation of brain endothelial cells using two computational strategies: Temporal Pathway Synthesizer (TPS), which uses phosphoproteomics data as input, and Temporally REsolved KInase Network Generation (TREKING), which uses kinase inhibitor screens. TPS and TREKING predicted overlapping barrier-regulatory kinases connected with unique network topology. Each strategy effectively describes regulatory signaling networks and is broadly applicable across biological systems.

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Wei, L., Mast, F. D., Aitchison, J. D., Kaushansky, A.. 2024-08-05. Systems-level reconstruction of kinase phosphosignaling networks regulating endothelial barrier integrity using temporal data. https://doi.org/10.1101/2024.08.01.606198

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