Genetic diversity and antimicrobial resistance of Campylobacter jejuni isolates from Gambian children under five with moderate-to-severe diarrhoea and healthy Controls

Betts, M.; Dione, M.; Ikumapayi, U. N.; Senghore, M.; Lamin, M.; Foster-Nyarko, E.; Jaffali, J.; Panchalingam, S.; Nataro, J. P.; Kotloff, K. L.; Levine, M. M.; Saha, D.; Hossain, J. M.; Kwambana-Adams, B.; Antonio, M.

doi:10.1101/2024.07.30.605890

bioRxiv · 10.1101/2024.07.30.605890

Genetic diversity and antimicrobial resistance of Campylobacter jejuni isolates from Gambian children under five with moderate-to-severe diarrhoea and healthy Controls

Betts, M.·Dione, M.·Ikumapayi, U. N.·Senghore, M.·Lamin, M.·Foster-Nyarko, E.·Jaffali, J.·Panchalingam, S.·Nataro, J. P.·Kotloff, K. L.·Levine, M. M.·Saha, D.·Hossain, J. M.·Kwambana-Adams, B.·Antonio, M.

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Abstract

IntroductionCampylobacter is a leading cause of bacterial gastroenteritis globally, but its molecular epidemiology remains poorly understood in sub-Saharan Africa. This study investigates the genotypic population structure of Campylobacter jejuni isolates from children with moderate-to-severe diarrhoea (MSD) and healthy controls in The Gambia. Additionally, we determined the antimicrobial susceptibility levels of the isolates. MethodsAs part of the Global Enteric Multicenter Study (GEMS) in The Gambia, a total of 49 C. jejuni isolates were collected from the stools of children under 5 years old, including 22 with MSD and 27 healthy controls. These isolates were subjected to multilocus sequence typing (MLST) and antimicrobial susceptibility testing using the disc-diffusion method. ResultsThe C. jejuni isolates belonged to 22 sequence types (STs), ten of which were novel. The most common STs were ST-353 (19.1%, 9/47), ST-7784 (12.7%, 6/47), and ST-1038 (10.6%, 5/47). All isolates were fully susceptible to erythromycin, tetracycline, gentamicin and chloramphenicol, with two isolates (4.4%, 2/45) resistant to ciprofloxacin and nalidixic acid. Antimicrobial resistance or intermediate susceptibility to trimethoprim-sulfamethoxazole, cefotaxime and ampicillin was observed in 91.1% (41/45), 90.9% (40/44), and 44.4% (20/45) of the isolates, respectively. There was no strong evidence linking C. jejuni antimicrobial susceptibility or MLST genotype to MSD status. ConclusionThis study provides the first overview of the high genotypic diversity of human C. jejuni isolates in The Gambia and reveals low-level resistance among the isolates to antibiotics commonly used to treat campylobacteriosis. The study contributes to understanding the epidemiology and resistance patterns of C. jejuni in this region.

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Betts, M., Dione, M., Ikumapayi, U. N., Senghore, M., Lamin, M., Foster-Nyarko, E., Jaffali, J., Panchalingam, S., Nataro, J. P., Kotloff, K. L., Levine, M. M., Saha, D., Hossain, J. M., Kwambana-Adams, B., Antonio, M.. 2024-08-02. Genetic diversity and antimicrobial resistance of Campylobacter jejuni isolates from Gambian children under five with moderate-to-severe diarrhoea and healthy Controls. https://doi.org/10.1101/2024.07.30.605890

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