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bioRxiv · 10.1101/2024.07.24.604972

Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins

Mamonska, M. D.·Basczok, M. M.·Stein, E. M.·Olejniczak, M.
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Abstract

Escherichia coli ProQ and FinO proteins both have RNA-binding FinO domains, which bind to intrinsic transcription terminators, but each protein recognizes distinct RNAs. To explore how ProQ and FinO discriminate between RNAs we transplanted sequences surrounding terminator hairpins between RNAs specific for each protein, and compared their binding to ProQ, the isolated FinO domain of ProQ (ProQNTD), and FinO. The results showed that the binding specificity of chimeric RNAs towards ProQ, ProQNTD, or FinO was determined by the origin of the transplanted sequence. Further analysis showed that the sequence surrounding the terminator hairpin, including a purine-purine mismatch, in natural RNA ligands of FinO and in chimeric RNAs weakened their binding by ProQNTD. Overall, our studies suggest that the discrimination between RNAs by ProQ and FinO is determined by RNA sequence elements surrounding the intrinsic terminator hairpin.

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Mamonska, M. D., Basczok, M. M., Stein, E. M., Olejniczak, M.. 2024-07-24. Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins. https://doi.org/10.1101/2024.07.24.604972

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