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bioRxiv · 10.1101/2024.07.21.604461

Mathematical modeling using box-behnken response surface design for optimal DNA yield with variable parameters of time, temperature, and proteinase K concentration

Afzal, F.·Zia, S.·Saif, R.
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Abstract

The current research aims to explore an enzyme-assisted inorganic DNA extraction protocol to obtain maximum yield from whole blood. We considered various operating conditions such as incubation temperature and time along with proteinase K enzyme concentration. To model and simulate the extraction process, Box-Behnken Design (BBD) was employed with three factors and its five levels, coupled with the desired functional response surface methodology (RSM). RSM entails fitting a mathematical model for experimental data to optimize a response variable with Box-Behnken Design. The best simulation settings yielded the maximum DNA at an incubation temperature of 56{degrees}C for 10 hours and proteinase K enzyme concentration of 10{micro}L. Utilizing these parameters, a yield of 300{micro}L was obtained. Furthermore, other DNA extraction parameters may also be added for a more robust design for obtaining the maximum DNA yield.

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Afzal, F., Zia, S., Saif, R.. 2024-07-21. Mathematical modeling using box-behnken response surface design for optimal DNA yield with variable parameters of time, temperature, and proteinase K concentration. https://doi.org/10.1101/2024.07.21.604461

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