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bioRxiv · 10.1101/2024.07.19.604387

A high-performance genetically encoded sensor for cellular imaging of PKC activity in vivo

Yahiro, T.·Bayless-Edwards, L.·Jones, J. A.·Ma, L.·Qin, M.·Mao, T.·Zhong, H.
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Abstract

We report a genetically encoded fluorescence lifetime sensor for protein kinase C (PKC) activity, named CKAR3, based on Forster resonance energy transfer. CKAR3 exhibits a 10-fold increased dynamic range compared to its parental sensors and enables in vivo imaging of PKC activity during animal behavior. Our results reveal robust PKC activity in a sparse neuronal subset in the motor cortex during locomotion, in part mediated by muscarinic acetylcholine receptors.

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Yahiro, T., Bayless-Edwards, L., Jones, J. A., Ma, L., Qin, M., Mao, T., Zhong, H.. 2024-07-23. A high-performance genetically encoded sensor for cellular imaging of PKC activity in vivo. https://doi.org/10.1101/2024.07.19.604387

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