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bioRxiv · 10.1101/2024.07.15.603557

COLLAGE: COnsensus aLignment of muLtiplexing imAGEs

Antoranz, A.·Arnould, A.·Andhari, M. D.·Nazari, P.·Shankar, G.·De Moor, B.·De Smet, F.·Bosisio, F. M.·Pey, J.
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Abstract

Multiplexed immunohistochemistry (mIHC) enables the high-dimensional single-cell interrogation of pathological tissue samples. mIHC is commonly based on the collection of high-resolution images from repeated staining cycles of the same tissue sample. Images of individual cycles typically consist of smaller tiles that need to be stitched into larger composite images, while images from serial rounds require alignment in a shared set of coordinates to enable pixel-perfect data integration. Current algorithms for stitching and registration require solving a single large puzzle consisting of billions of pixels making them computationally expensive but moreover forcing them to introduce errors to close the puzzle, which significantly impact the downstream results and the single-cell profiles. Here, we present the development and evaluation of COLLAGE (COnsensus aLignment of muLtiplexing imAGEs), an innovative stitching and registration method that leverages on the complementarity of these two steps in a divide and conquer approach: in contrast to other algorithms, COLLAGE breaks the process down into thousands of small puzzles, enabling extensive parallelisation and not forcing errors in its solution. Because COLLAGE also includes AlgnQC, a novel deep-learning-based evaluation metric of registration quality, the quality of the resulting image stacks is consistently maximised, while images with errors are flagged in an automated way. COLLAGE is available via www.disscovery.org. O_FIG O_LINKSMALLFIG WIDTH=148 HEIGHT=200 SRC="FIGDIR/small/603557v1_ufig1.gif" ALT="Figure 1"> View larger version (65K): org.highwire.dtl.DTLVardef@18d0f5borg.highwire.dtl.DTLVardef@1eb2756org.highwire.dtl.DTLVardef@163bb95org.highwire.dtl.DTLVardef@b04453_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Antoranz, A., Arnould, A., Andhari, M. D., Nazari, P., Shankar, G., De Moor, B., De Smet, F., Bosisio, F. M., Pey, J.. 2024-07-17. COLLAGE: COnsensus aLignment of muLtiplexing imAGEs. https://doi.org/10.1101/2024.07.15.603557

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