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bioRxiv · 10.1101/2024.07.03.601914

A DIY guide for image-based spatial transcriptomic: TLS as a case example

Defard, t.·DESRENTES, A.·Fouillade, C.·Mueller, F.
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Abstract

Spatial RNA profiling methods provide insight to the cellular heterogeneity and spatial architecture of complex, multi-cellular systems. Combining molecular and spatial information provides important clues to study tissue architecture in development and disease. Here, we present a comprehensive do-it-yourself guide to perform such experiments at reduced costs leveraging open-source approaches. This guide spans the entire life cycle of a project, from its initial definition to experimental choices, wet lab approaches, instrumentation and analysis. As a concrete example, we focus on Tertiary lymphoid structures (TLS), which we use to develop typical questions that can be addressed by these approaches.

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Defard, t., DESRENTES, A., Fouillade, C., Mueller, F.. 2024-07-05. A DIY guide for image-based spatial transcriptomic: TLS as a case example. https://doi.org/10.1101/2024.07.03.601914

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