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bioRxiv · 10.1101/2024.06.30.601363

FLT3 is genetically essential for ITD-mutated leukemic stem cells but dispensable for human hematopoietic stem cells

Araujo, J. L.·Wagenblast, E.·Voisin, V.·McLeod, J.·Gan, O. I.·Bansal, S.·Jin, L.·Mitchell, A.·Gratton, B.·Cutting, S.·Arruda, A.·Doedens, M.·Capo-Chichi, J.-M.·Abelson, S.·Minden, M. D.·Wang, J. C.·Sobrinho-Simoes, M. A.·Pinto-do-O, P.·Lechman, E.·Dick, J. E.
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Abstract

Leukemic stem cells (LSCs) fuel acute myeloid leukemia (AML) growth and relapse, but therapies tailored towards eradicating LSCs without harming healthy hematopoietic stem cells (HSCs) are lacking. FLT3 is considered an important therapeutic target due to frequent mutation in AML and association with relapse. However, there has been limited clinical success with FLT3 targeting, suggesting either that FLT3 is not a vulnerability in LSC, or that more potent inhibition is required, a scenario where HSC toxicity could become limiting. We tested these possibilities by ablating FLT3 using CRISPR/Cas9-mediated FLT3 knock-out (FLT3-KO) in human LSCs and HSCs followed by functional xenograft assays. FLT3-KO in LSCs from FLT3-ITD mutated, but not FLT3-WT AMLs, resulted in short-term leukemic grafts of FLT-3-KO edited cells that disappeared by 12 weeks. By contrast, FLT3-KO in HSCs from fetal liver, cord blood and adult bone marrow did not impair multilineage hematopoiesis in primary and secondary xenografts. Our study establishes FLT3 as an ideal therapeutic target where ITD+ LSC are eradicated upon FLT3 deletion, while HSCs are spared. These findings support the development of more potent FLT3-targeting drugs or gene-editing approaches for LSC eradication to improve clinical outcomes. KEY POINTSThe FLT3 gene is essential for ITD-mutated leukemic stem cells (LSCs) to establish and propagate leukemia. Normal human hematopoietic stem cells (HSCs) do not require FLT3 to engraft and sustain hematopoiesis. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=179 SRC="FIGDIR/small/601363v1_ufig1.gif" ALT="Figure 1"> View larger version (51K): org.highwire.dtl.DTLVardef@b77f56org.highwire.dtl.DTLVardef@109011dorg.highwire.dtl.DTLVardef@282e04org.highwire.dtl.DTLVardef@1e51e3b_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Araujo, J. L., Wagenblast, E., Voisin, V., McLeod, J., Gan, O. I., Bansal, S., Jin, L., Mitchell, A., Gratton, B., Cutting, S., Arruda, A., Doedens, M., Capo-Chichi, J.-M., Abelson, S., Minden, M. D., Wang, J. C., Sobrinho-Simoes, M. A., Pinto-do-O, P., Lechman, E., Dick, J. E.. 2024-07-02. FLT3 is genetically essential for ITD-mutated leukemic stem cells but dispensable for human hematopoietic stem cells. https://doi.org/10.1101/2024.06.30.601363

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