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bioRxiv · 10.1101/2024.06.25.600609

High efficient chromatin conformation capture without pre-enrichment (HiChew) in single cells

Tang, C.·Chen, Z.·Xie, Y.·Tan, C.·Ruan, F.·Luo, M.·Zhang, C.·Guo, M.·Fang, Y.
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Abstract

This study presents HiChew, a cutting-edge technique for high-efficiency chromatin conformation capture in single cells, without the need for pre-enrichment. This unique approach minimizes the risk of cell or DNA loss. When compared to Dip-C, HiChew captures valid pairs with 4-8 times more efficiency, reducing wastage and saving significant sequencing budget. Furthermore, HiChew delivers a lower false positive ratio, ensuring data accuracy. It also achieves more contacts per cell, enhancing resolution in single cell. HiChews superior performance not only enhances single-cell Hi-C but also streamlines conventional Hi-C, making it more robust than conventional HiC methods. This study also unveils a fascinating mechanism of gene activation in the B compartment of chromatin, providing insight into the elusive aspect of gene expression within this region.

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Tang, C., Chen, Z., Xie, Y., Tan, C., Ruan, F., Luo, M., Zhang, C., Guo, M., Fang, Y.. 2024-06-29. High efficient chromatin conformation capture without pre-enrichment (HiChew) in single cells. https://doi.org/10.1101/2024.06.25.600609

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