Metabolic analysis in intact human-derived cerebral organoids by high-resolution magic-angle spinning NMR spectroscopy

Castilla Bolanos, M. A.; Chinchalongporn, V.; Ghosh Biswas, R.; Bailey, C.; Wu, M.; Soong, R.; Saleh, F.; Simpson, A.; Schuurmans, C.; Near, J.

doi:10.1101/2024.06.21.599412

bioRxiv · 10.1101/2024.06.21.599412

Metabolic analysis in intact human-derived cerebral organoids by high-resolution magic-angle spinning NMR spectroscopy

Castilla Bolanos, M. A.·Chinchalongporn, V.·Ghosh Biswas, R.·Bailey, C.·Wu, M.·Soong, R.·Saleh, F.·Simpson, A.·Schuurmans, C.·Near, J.

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Abstract

Human-derived cerebral organoids (COs) are an emerging model system for the study of human neural development and physiology. Here, we describe the assessment of metabolism in human-derived COs using high-resolution magic-angle spinning (HR-MAS) NMR spectroscopy. Metabolic changes during development are assessed by studying COs at various stages of maturity. Our results suggest that COs exhibit a metabolic profile similar to in vivo human brain metabolism, albeit with a few notable metabolic differences.

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Castilla Bolanos, M. A., Chinchalongporn, V., Ghosh Biswas, R., Bailey, C., Wu, M., Soong, R., Saleh, F., Simpson, A., Schuurmans, C., Near, J.. 2024-06-23. Metabolic analysis in intact human-derived cerebral organoids by high-resolution magic-angle spinning NMR spectroscopy. https://doi.org/10.1101/2024.06.21.599412

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